Comparing experimental with predicted parameters
Parameters predicted by bead models can be compared with those determined
experimentally using techniques such as AUC, DLS, NMR, FS and SAS (provided
that the latter parameters have been corrected for standard conditions). The
closer the comparison, the more plausible is the model. However, bead models
cannot be used to define unique structures, since different models could have
similar overall parameters. Therefore bead modelling in this sense is better
used as method that will filter out implausible structures. Obviously
confidence in structural prediction using solution techniques will improve
with the application of more filters. This is one of the reasons why there is
an interest in combining hydrodynamic and Debye sphere modelling strategies.